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Phage display technology fuses and displays exogenous proteins, single-domain antibodies, or peptide sequences on the surface of M13 bacteriophage coat proteins, achieving phenotype-genotype linkage. Through multiple rounds of affinity biopanning, specific binding molecules targeting desired antigens can be rapidly obtained. This technology is widely applied in antibody drug discovery, diagnostic reagent development, protein-protein interaction studies, epitope mapping, and peptide ligand discovery.

Leveraging our mature phage display platform, ChinaPeptides (QYAOBIO) provides screening services for fully human single-domain antibody (sdAb) library naive libraries, alpaca nanobody naive libraries, and peptide libraries. Clients can directly initiate biopanning projects without building custom libraries, significantly shortening the R&D cycle.

Phage Display Fully Fuman Single-Domain Antibody (SDAB) Library Naive Library Screening

Immunization-free in vitro screening for low‑immunogenicity antibodies, tailored for therapeutic and diagnostic antibody development.

Phage Display Alpaca Nanobody Naive Library Screening

High stability and superior tissue penetration, capable of recognizing cryptic epitopes; ideal for academic research, diagnostics, and antibody drug discovery.

Phage Display Peptide Library Screening

High-throughput biopanning of target-specific peptides for epitope mapping, ligand discovery, and peptide inhibitor development.

Off-the-Shelf High-Capacity Libraries
Pre-made, large-capacity libraries eliminate time-consuming library construction.
Full-Process Quality Control
Strict quality control throughout the workflow ensures fully traceable and reliable data.
One-Stop Service
End-to-end service coverage eliminates the hassle of coordinating with multiple vendors.
Professional Technical Team
Decades of deep industry expertise backed by extensive project experience.
Flexible Delivery Options
Customized deliverables tailored to satisfy diverse project requirements.
1 Project Assessment & Strategy Confirmation
2 Antigen Preparation & Pretreatment
3Multi-round Phage Affinity Biopanning
4 Single Colony Picking & Primary Screening/Validation
5Sequencing & Sequence Analysis
6Candidate Molecule Validation
7Project Completion & Final Delivery
A1: For protein antigens, a purity of >80% with intact native conformation is recommended, and the amount should ideally be ≥100 μg. For cellular targets, stably cultured cells must be provided. Antigen quality directly determines screening success; we can also assist with antigen labeling and quality control.
A2: Typically, 3–5 rounds of biopanning are performed. Results vary depending on the target; under standard conditions, 5–20 non-redundant candidate sequences can be identified. Rather than promising a fixed number, final counts are based on actual experimental enrichment results.
A3: Yes. ChinaPeptides possesses multiple recombinant protein expression and purification platforms—including E. coli, Bacillus subtilis, mammalian cells, Pichia pastoris, and insect baculovirus systems—to fulfill diverse recombinant protein preparation requirements.
A4:Standard Deliverables: Final experimental report, raw sequencing data, and a de-duplicated candidate sequence list.Optional Deliverables: Positive plasmids / glycerol stocks, small-scale expressed proteins, and affinity measurement reports.
A5: Phage ELISA only accomplishes preliminary specificity screening. Functional parameters such as binding affinity, neutralizing activity, and inhibitory activity require downstream expression and characterization, which can be provided as an optional follow-up service.
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