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Peptide Synthesis Resource

Peptide Synthesis Technologies
·Introduction
·Solid Phase Peptide Synthesis (SPPS)
·Fmoc Strategy in SPPS
· Boc Strategy in SPPS
·General Coupling Methods in SPPS
·Solution Phase Synthesis
· HPLC Analysis and Purification
·Handling and Storage of Peptides
·References

Tips for Peptide Synthesis

·Reconstitution and Storage of Peptides
· Methods to Dissolve Peptides
·Preparation of HBTU/HOBt Solution for the Peptide Synthesizer
·Biotinylation of Amino Group
·Procedure for Loading Fmoc-Amino Acid to 2-Chlorotrityl Chloride Resin
·Procedure for Checking Substitution of Fmoc-Amino Acid Loaded Resins
· Manual Fmoc Synthesis (0.25 mmol)
·Synthesis of Phosphotyrosine-Containing Peptides Using Fmoc-Phosphotyrosine
·Simultaneous Synthesis of Peptides Which Differ in the C-Termini Using 2-Chlorotrityl Resin and Wang Resin
·Cleavage Protocol to Produce Fully Protected Peptide
·Procedure for Removing Mtt group from Fmoc-Lys(Mtt) on Solid Phase
·Procedure for FITC Labeling of Peptides
·Procedure for Fluorescein Labeling of Peptides

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