| QY-2001 | Protein → McAb | The customer provides 2-5mg protein (purity>85%, concentration>0.5mg/ml). Quality inspection of protein: 1. Four SPF Balb/c female mice were immunized, and each mouse was immunized 4-5 times. The serum Elisa titer of the mice reached more than 1:10000< br> 2. Take the spleen cells of the mouse with the highest serum titer for cell fusion with SP2/0 or send the immunized serum to the customer for verification, and then the customer will designate one mouse for fusion< br> 3. Subcloning and obtaining hybridoma cell line stably secreting specific antibody after multiple rounds of ELISA screening< br> 4. Hybridoma cell line expansion/cryopreservation< br> 5. The positive supernatant was sent to the customer for verification, and the cell strain designated by the customer was used to prepare mouse ascites, and Protein A/G was purified to obtain antibodies< br> 6. Antibody Elisa test titer, subtype identification, WB test, and affinity constant test data. |
Final delivery of materials includes: 1. Positive cell lines: at least 1-5 hybridoma cell lines (many donated); 2. Positive cell supernatant: at least 3-10 strains (more than gift); 3.Protein A/G affinity purification: provide 5mg purified monoclonal | 16-24 weeks |
| QY-2002 | Polypeptide → McAb | The customer provides 10mg polypeptide (purity>85%), peptide coupling: 1. Four SPF Balb/c female mice were immunized, and each mouse was immunized 4-5 times. The serum Elisa titer of the mice reached more than 1:10000; 2. Take the spleen cells of the mouse with the highest serum titer for cell fusion with SP2/0 or send the immunized serum to the customer for verification, and then the customer will designate one mouse for fusion< br> 3. Subcloning and obtaining hybridoma cell line stably secreting specific antibody after multiple rounds of ELISA screening< br> 4. Hybridoma cell line expansion/cryopreservation< br> 5. The positive supernatant was sent to the customer for verification, and the cell strain designated by the customer was used to prepare mouse ascites, and Protein A/G was purified to obtain antibodies< br> 6. Antibody Elisa test titer, subtype identification, WB test, and affinity constant test data. |
The final delivered materials include: 1. Positive cell lines: at least 3-10 (many will be given as gifts)< br> 2. Positive cell supernatant: at least 3-10 strains (many will be given as gifts)< br> 3. Protein A/G affinity purification: provid |
16-24 weeks |
| QY-2003 | Modified polypeptide → monoclonal antibody (Phosphorylation/methylation/acetylation/succinylation/ubiquitination modification, etc.) | The customer provides 15 mg of modified polypeptide (purity>85-95%), 10 mg of unmodified polypeptide (purity>85%), peptide coupling (KLH coupled modified polypeptide for immunity, BSA coupled modified polypeptide for screening, BSA coupled unmodified polypeptide for negative screening): 1. Four SPF Balb/c female mice were immunized, and each mouse was immunized 4-5 times. The serum Elisa titer of the mice reached more than 1:10000< br> 2. Take the spleen cells of the mouse with the highest serum titer for cell fusion with SP2/0 or send the immunized serum to the customer for verification, and then the customer will designate one mouse for fusion< br> 3. Subcloning and obtaining hybridoma cell line stably secreting specific antibody after multiple rounds of ELISA screening< br> 4. Hybridoma cell line expansion/cryopreservation< br> 5. The positive supernatant was sent to the customer for verification, and the cell strain designated by the customer was used to prepare mouse ascites, and Protein A/G was purified to obtain antibodies< br> 6. Antibody Elisa test titer, subtype identification, WB test, and affinity constant test data. |
The final delivered materials include: 1. Positive cell lines: at least 1-3 (many will be given as gifts)< br> 2. Positive cell supernatant: at least 1-3 strains (many will be given as gifts)< br> 3. Protein A/G affinity purification: provide |
16-24 weeks |
| QY-2004 | Small molecule compound → monoclonal antibody | The customer provides 10-30mg of small molecules (it is required that small molecules have complex structures and must preferably have carboxyl groups, followed by amino groups, with purity>90%), and small molecules are coupled: 1. Four SPF Balb/c female mice were immunized, and each mouse was immunized 4-5 times. The serum Elisa titer of the mice reached more than 1:10000< br> 2. Take the spleen cells of the mouse with the highest serum titer for cell fusion with SP2/0 or send the immunized serum to the customer for verification, and then the customer will designate one mouse for fusion< br> 3. Subcloning and obtaining hybridoma cell line stably secreting specific antibody after multiple rounds of ELISA screening< br> 4. Hybridoma cell line expansion/cryopreservation< br> 5. The positive supernatant was sent to the customer for verification, and the cell strain designated by the customer was used to prepare mouse ascites, and Protein A/G was purified to obtain antibodies< br> 6. Antibody Elisa test titer, subtype identification, WB test, and affinity constant test data. |
The final delivered materials include: 1. Positive cell lines: at least 1-3 (many will be given as gifts)< br> 2. Positive cell supernatant: at least 1-3 strains (many will be given as gifts)< br> 3. Protein A/G affinity purification: provide |
16-24 weeks |